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Mean values (± SEM) of total motility of ram spermatozoa immediately after <t>UVB</t> exposure (0 h – blue), and after 24 h (green) and 48 h (red) of cold storage at 5 °C. Sperm samples were evaluated following exposure to <t>UVB</t> <t>radiation</t> at different time intervals (0, 30, 60, 90, 120, and 150 s). Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05) within each storage timepoint, i.e., differences between exposure times at 0 h, 24 h, and 48 h, respectively. Radiation 0 s = 0; 30 s = 2.199 mJ/cm2; 60 s = 4.398 mJ/cm2; 90 s = 6.597 mJ/cm2; 120 s = 8.796 mJ/cm2; 150 s = 10.995 mJ/cm2
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thin layer chromatography tlc uv lamp  (VILBER GmbH)
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Mean values (± SEM) of total motility of ram spermatozoa immediately after <t>UVB</t> exposure (0 h – blue), and after 24 h (green) and 48 h (red) of cold storage at 5 °C. Sperm samples were evaluated following exposure to <t>UVB</t> <t>radiation</t> at different time intervals (0, 30, 60, 90, 120, and 150 s). Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05) within each storage timepoint, i.e., differences between exposure times at 0 h, 24 h, and 48 h, respectively. Radiation 0 s = 0; 30 s = 2.199 mJ/cm2; 60 s = 4.398 mJ/cm2; 90 s = 6.597 mJ/cm2; 120 s = 8.796 mJ/cm2; 150 s = 10.995 mJ/cm2
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Mean values (± SEM) of total motility of ram spermatozoa immediately after UVB exposure (0 h – blue), and after 24 h (green) and 48 h (red) of cold storage at 5 °C. Sperm samples were evaluated following exposure to UVB radiation at different time intervals (0, 30, 60, 90, 120, and 150 s). Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05) within each storage timepoint, i.e., differences between exposure times at 0 h, 24 h, and 48 h, respectively. Radiation 0 s = 0; 30 s = 2.199 mJ/cm2; 60 s = 4.398 mJ/cm2; 90 s = 6.597 mJ/cm2; 120 s = 8.796 mJ/cm2; 150 s = 10.995 mJ/cm2

Journal: International Journal of Biometeorology

Article Title: Effects of ultraviolet B radiation and cold storage on Ram sperm morphology and physiology

doi: 10.1007/s00484-025-03082-4

Figure Lengend Snippet: Mean values (± SEM) of total motility of ram spermatozoa immediately after UVB exposure (0 h – blue), and after 24 h (green) and 48 h (red) of cold storage at 5 °C. Sperm samples were evaluated following exposure to UVB radiation at different time intervals (0, 30, 60, 90, 120, and 150 s). Different lowercase letters above the bars indicate statistically significant differences ( p < 0.05) within each storage timepoint, i.e., differences between exposure times at 0 h, 24 h, and 48 h, respectively. Radiation 0 s = 0; 30 s = 2.199 mJ/cm2; 60 s = 4.398 mJ/cm2; 90 s = 6.597 mJ/cm2; 120 s = 8.796 mJ/cm2; 150 s = 10.995 mJ/cm2

Article Snippet: Radiation was delivered using a UVB lamp (VL-115 C, 30 W, peak emission at 312 nm; Vilber Lourmat, Marne Lavallée, France), positioned at a fixed distance from the samples.

Techniques:

presents data on the structural integrity of ram spermatozoa after exposure to UVB radiation at different time intervals, assessed using epifluorescence microscopy. The figure includes results obtained immediately after exposure (0 h), as well as after 24 h and 48 h of cold storage at 5 °C. The analysis revealed no statistically significant DNA damage across treatments. However, UVB exposure led to a reduction in acrosomal and mitochondrial integrity, with the most pronounced damage observed after 150 s (10.995 mJ/cm²) of exposure. Moreover, the data indicate that the structural damage occurred at the moment of exposure and remained stable during the subsequent storage periods (24 h and 48 h), with no evidence of progression or recovery over time.10.995

Journal: International Journal of Biometeorology

Article Title: Effects of ultraviolet B radiation and cold storage on Ram sperm morphology and physiology

doi: 10.1007/s00484-025-03082-4

Figure Lengend Snippet: presents data on the structural integrity of ram spermatozoa after exposure to UVB radiation at different time intervals, assessed using epifluorescence microscopy. The figure includes results obtained immediately after exposure (0 h), as well as after 24 h and 48 h of cold storage at 5 °C. The analysis revealed no statistically significant DNA damage across treatments. However, UVB exposure led to a reduction in acrosomal and mitochondrial integrity, with the most pronounced damage observed after 150 s (10.995 mJ/cm²) of exposure. Moreover, the data indicate that the structural damage occurred at the moment of exposure and remained stable during the subsequent storage periods (24 h and 48 h), with no evidence of progression or recovery over time.10.995

Article Snippet: Radiation was delivered using a UVB lamp (VL-115 C, 30 W, peak emission at 312 nm; Vilber Lourmat, Marne Lavallée, France), positioned at a fixed distance from the samples.

Techniques: Epifluorescence Microscopy